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Exogenous treatment with <t>Jasmonic</t> acid effectively stimulates the production of PTX. A . The level of JA in <t>the</t> <t>TmLOX13</t> -silenced cells compared to control cells. B. The amount of secreted PTX in the medium of TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated. C . Relative expression of key genes involved in PTX biosynthesis ( TXS , DBAT and DBTNBT ) in both TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated on days 8, 12, 16, 20 and 24 after inoculation (DAI). The color scale (black-red-white-green-black) indicates the relative changes of transcript abundance from − 20 to + 20-fold. All measurements were performed in triplicate. Data are mean values ± SD ( n = 3). Statistical significance was evaluated by ANOVA analysis. Asterisks indicate significant differences between JA-treated samples and their respective control samples ( * P < 0.05).
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Exogenous treatment with <t>Jasmonic</t> acid effectively stimulates the production of PTX. A . The level of JA in <t>the</t> <t>TmLOX13</t> -silenced cells compared to control cells. B. The amount of secreted PTX in the medium of TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated. C . Relative expression of key genes involved in PTX biosynthesis ( TXS , DBAT and DBTNBT ) in both TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated on days 8, 12, 16, 20 and 24 after inoculation (DAI). The color scale (black-red-white-green-black) indicates the relative changes of transcript abundance from − 20 to + 20-fold. All measurements were performed in triplicate. Data are mean values ± SD ( n = 3). Statistical significance was evaluated by ANOVA analysis. Asterisks indicate significant differences between JA-treated samples and their respective control samples ( * P < 0.05).
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Exogenous treatment with Jasmonic acid effectively stimulates the production of PTX. A . The level of JA in the TmLOX13 -silenced cells compared to control cells. B. The amount of secreted PTX in the medium of TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated. C . Relative expression of key genes involved in PTX biosynthesis ( TXS , DBAT and DBTNBT ) in both TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated on days 8, 12, 16, 20 and 24 after inoculation (DAI). The color scale (black-red-white-green-black) indicates the relative changes of transcript abundance from − 20 to + 20-fold. All measurements were performed in triplicate. Data are mean values ± SD ( n = 3). Statistical significance was evaluated by ANOVA analysis. Asterisks indicate significant differences between JA-treated samples and their respective control samples ( * P < 0.05).

Journal: Scientific Reports

Article Title: A new 13-lipoxygenase from Taxus media callus is functionally linked to paclitaxel biosynthesis

doi: 10.1038/s41598-025-26256-x

Figure Lengend Snippet: Exogenous treatment with Jasmonic acid effectively stimulates the production of PTX. A . The level of JA in the TmLOX13 -silenced cells compared to control cells. B. The amount of secreted PTX in the medium of TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated. C . Relative expression of key genes involved in PTX biosynthesis ( TXS , DBAT and DBTNBT ) in both TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated on days 8, 12, 16, 20 and 24 after inoculation (DAI). The color scale (black-red-white-green-black) indicates the relative changes of transcript abundance from − 20 to + 20-fold. All measurements were performed in triplicate. Data are mean values ± SD ( n = 3). Statistical significance was evaluated by ANOVA analysis. Asterisks indicate significant differences between JA-treated samples and their respective control samples ( * P < 0.05).

Article Snippet: Elicitation of both TmLOX13-silenced and wild-type T. media cells was carried out using jasmonic acid (purity > 98%, Larodan, Inc., Sweden) at the onset of the cell growth stage and maintained for 24 days .

Techniques: Control, Expressing